AllBestEssays.com - All Best Essays, Term Papers and Book Report
Search

Biology

Essay by   •  March 19, 2016  •  Study Guide  •  486 Words (2 Pages)  •  1,396 Views

Essay Preview: Biology

Report this essay
Page 1 of 2

SHALINI SUNDRAMURTHI CHELLIAH

27208664

PRACTICAL 1

               Table 1: Experimental protocol for construction of the protein standard curve

TUBE

1

2

3

4

5

6

Buffer (ml)

1.0

0.8

0.6

0.4

0.2

0.0

BSA Protein solution

(10mg ml-1)      (ml)

0.00

0.2

0.4

0.6

0.8

10.0

Biuret reagent   (ml)

4.0

4.0

4.0

4.0

4.0

4.0

Total volume   (ml)

5.0

5.0

5.0

5.0

5.0

5.0

Final protein concentration  

(mg ml-1)

0.0

2.0

4.0

6.0

8.0

10.0

Absorbance

0.0

0.08

0.18

0.27

0.35

0.45

Table2: Experimental protocol for determining the protein concentration of silverbeet grown in the sun and shade

Sun

Shade

Columns for modified procedure(s)

Procedure

Protein solution (ml)

1.0

1.0

0.25

0.25

Biuret reagent (ml)

4.0

4.0

0.75

0.75

Absorbance

1.05

0.87

0.20

0.15

Protein concentration derived

from standard curve (mg ml-1)

[pic 1][pic 2]

[pic 3][pic 4]

4.45

3.35

Protein concentration of original,

Unmodified solution (mg ml-1)

[pic 5][pic 6]

[pic 7][pic 8]

8.00

13.40

SHALINI SUNDRAMURTHI CHELLIAH

27208664

PART 1

F1.

[pic 9]

Figure1. Determination of the protein concentration of unknown solutions using standard curve

  1. Line of best fit should be drawn. This is because all the residual plots are along the line of  best fit.

SHALINI SUNDRAMURTHI CHELLIAH

...

...

Download as:   txt (3.6 Kb)   pdf (123 Kb)   docx (463.8 Kb)  
Continue for 1 more page »
Only available on AllBestEssays.com