Biology
Essay by SHALINI Sundramurthi • March 19, 2016 • Study Guide • 486 Words (2 Pages) • 1,372 Views
SHALINI SUNDRAMURTHI CHELLIAH
27208664
PRACTICAL 1
Table 1: Experimental protocol for construction of the protein standard curve
TUBE | ||||||
1 | 2 | 3 | 4 | 5 | 6 | |
Buffer (ml) | 1.0 | 0.8 | 0.6 | 0.4 | 0.2 | 0.0 |
BSA Protein solution (10mg ml-1) (ml) | 0.00 | 0.2 | 0.4 | 0.6 | 0.8 | 10.0 |
Biuret reagent (ml) | 4.0 | 4.0 | 4.0 | 4.0 | 4.0 | 4.0 |
Total volume (ml) | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 |
Final protein concentration (mg ml-1) | 0.0 | 2.0 | 4.0 | 6.0 | 8.0 | 10.0 |
Absorbance | 0.0 | 0.08 | 0.18 | 0.27 | 0.35 | 0.45 |
Table2: Experimental protocol for determining the protein concentration of silverbeet grown in the sun and shade
Sun | Shade | Columns for modified procedure(s) | ||
Procedure | ||||
Protein solution (ml) | 1.0 | 1.0 | 0.25 | 0.25 |
Biuret reagent (ml) | 4.0 | 4.0 | 0.75 | 0.75 |
Absorbance | 1.05 | 0.87 | 0.20 | 0.15 |
Protein concentration derived from standard curve (mg ml-1) | [pic 1][pic 2] | [pic 3][pic 4] | 4.45 | 3.35 |
Protein concentration of original, Unmodified solution (mg ml-1) | [pic 5][pic 6] | [pic 7][pic 8] | 8.00 | 13.40 |
SHALINI SUNDRAMURTHI CHELLIAH
27208664
PART 1
F1.
[pic 9]
Figure1. Determination of the protein concentration of unknown solutions using standard curve
- Line of best fit should be drawn. This is because all the residual plots are along the line of best fit.
SHALINI SUNDRAMURTHI CHELLIAH
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